At present, the available diagnostic kits such as NS1 and IgM kits have been designed to diagnose symptomatic dengue patients. However, the main carrier in spreading the dengue virus is attributable to individuals with no noticeable of the diseases (asymptomatic). The virus is primarily spread via human transmission (blood donation) and mosquito transmission. Hence, it is an urgent need to reduce/stop the dengue transmission by providing a rapid and sensitive method to diagnose asymptomatic people, who do not seek treatment from a doctor. Herein, surface plasmon resonance (SPR) sensor based on RGO-PAMAM thin film is developed for detection of DENV E-proteins.

The present innovation is to provide a rapid and sensitive technique to detect the presence of the dengue virus among asymptomatic people. The technique comprises the integrated RGO-PAMAM thin film with SPR sensor in order to detect dengue virus E-proteins (DENV E-proteins). To the best of our knowledge, this is first study on detection of DENV E-proteins. It has been proven that the detection limit was 0.08 pM within 8 min only.

Inventor
Dr. Yap Wing Fen
Faculty of Science
yapwingfen@upm.edu.my

Date of Input: 05/12/2020 | Updated: 05/12/2020 | asrizam