A Platform For Lipase Expression In Yeast System | PUTRA SCIENCE PARK
» ARTICLE » A platform for lipase expression in yeast system

A platform for lipase expression in yeast system

LipExPS is a web-based tool which uses a newly curated database (LipExDB) to provide an effective platform for predicting the best promoter/ host combination to express lipases in Pichia pastoris, which can be subsequently used in various industries.

 

CURRENT ISSUES

  • Pichia pastoris as the most conventional yeast expression host with various strains and promoters (18 combinations).
  • Time-consuming and expensive trial-and-error experiments to develop an optimum lipase-expressing P. pastoris system with suitable promoter/ host combination.
  • Suboptimal promoter/ host combination leads to excessive consumption of hazardous compounds like methanol and methylamine.
  • LipExPS is a novel platform which can be used for energy saving to generate the best recombinant lipase expression in yeast.
  • This web-based tool can reduce the use of non-environmentally friendly methanol/ methylamine consumption.

 

IMPACT OF THE PRODUCT

  • Reduced the wastage of hazardous compounds at the early cloning stage.
  • Lower developmental cost for Pichia pastoris system with an optimum lipase expression at a much-shortened time.
  • Minimized production cost and selling price of lipase products.
  • Earlier market entry of lipase products.

 

INVENTOR

Assoc. Prof Dr. Siti Nurbaya Oslan
Faculty of Biotechnology and Biomolecular Sciences
snurbayaoslan@upm.edu.my

Date of Input: 26/02/2021 | Updated: 26/02/2021 | asrizam

MEDIA SHARING

PUTRA SCIENCE PARK
Universiti Putra Malaysia
43400 UPM Serdang
Selangor Darul Ehsan
03-97691849
03-89464121
W1UKSTA4:19:40